Movie legend Marilyn Monroe was a fan after famously saying she wore the perfume in bed. Photo: AP
The scent is said to have got its name after designer Coco Chanel was presented with several samples to choose from, each labelled with a number.
After choosing the fifth bottle, she declared that the perfume would simply be called No.5.
Now, the popular jasmine scent, which first went on sale in 1921, is thought to sell a bottle every 55 seconds around the world.
Even movie legend Marilyn Monroe was a fan after famously saying she wore the perfume in bed.
Matt Twigg, fragrance buyer for Superdrug, said: "Perfume is such an essential part of getting ready for a woman and the fragrance they choose can really reflect their mood at that moment.
"Chanel No.5 is an iconic perfume which has truly stood the test of time, being just as popular now as when it first went on sale almost 90 years ago.
"All the perfumes in the top 10 have adorned dressing tables across the country and will remain staple scents for years to come.
"It's good to see a celebrity perfume making it into the list from the Beckhams too - they are the ultimate celebrity couple and their fragrance is just as sexy as their advertising shots."
Calvin Klein scooped second and third place in the poll of 2,000 women by Superdrug with their unisex perfume One and iconic scent Eternity.
Chanel also featured for a second time, in fourth place, with her scent Coco, which was launched in honour of the designer following her death.
Dior's Poison was at five, followed by Ghost's original scent, Ghost and Cacharel's Anais Anais, which was launched in 1979.
Eighth place went to Lacoste and Pink, followed by Yves Saint Lauren's Opium.
David and Victoria Beckham are the only celebrities to have a scent feature in the poll, with Beckham - Intimately Her completing the top ten.
The perfume, which first went on sale in 2006 was launched alongside the male equivalent, Intimately His.
Top ten most iconic perfumes of all time
1. Chanel No 5
2. Calvin Klein - One
3. Calvin Klein - Eternity
4. Chanel - Coco
5. Dior - Poison
7. Cacharel - Anais Anais
8. Lacoste - Pink
9. Yves Saint Lauren - Opium
10. Beckham - Intimately Her
Chanel No. 5 most iconic perfurme - Telegraph